Revealing the Conformational Preferences of Proteinogenic Glutamic Acid Derivatives in Solution by 1 H NMR Spectroscopy and Theoretical Calculations

publication · 4 months ago
by Weslley G. D. P. Silva, Claudio F. Tormena, Roberto Rittner (University of Campinas, University of Manitoba)
Marvin Sketch

The conformational preferences of proteinogenic glutamic acid esterified (GluOMe) and N-acetylated (Ac-GluOMe) derivatives have been determined in solution for the first time. Theoretical calculations at the ωB97X-D/aug-cc-pVTZ made possible the assignment of six and eight stable conformers for GluOMe and AcGluOMe, respectively. The conformational equilibrium of the studied compounds was evaluated in different organic solvents using a combination of the integral equation formalism polarizable continuum model (IEF-PCM) and 1 H NMR spectroscopy data. The results showed that the conformational equilibrium of both derivatives change in the presence of solvent. According to the quantum theory of atoms in molecules (QTAIM), non-covalent interactions (NCI), and natural bond orbitals (NBO) analyses, the conformational preferences observed for GluOMe and AcGluOMe are not dictated by the presence of a specific interaction but are due to a combination of hyperconjugative and steric effects.

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