High-Throughput Screening for Identification of Inhibitors of the Bacterial Phosphatase YopH
Our main goal was to establish a biochemical platform from assay setup to data management in order to set new standards for the service offered by LCBU. As target we chose tyrosine phosphatase (YopH)[1] which is one of the virulence factors produced by the Gram-negative bacterium Yersinia pseudotuberculosis. The set-up was optimized for assays in 384w plates.